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Trypsin 0.5 ukatal/mg
CAS 9002-07-7
Source Porcine (Bovine) pancreas
General description
Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23300 dalton, and is a single peptide chain composed of 223 amino acid residues. With rigorous specificity, the trypsin specifically acts on the peptide linkage constituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation also reduces or loses gradually. It is easily soluble in the water, but insoluble in trichloromethane, ethanol, ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated when boiled for a short time; if the salt is added into the hot solution, the protein will precipitate, and the enzyme action of filtrate cannot be seen, and Ca2+ plays the role in protecting and activating the trypsin.
The high purity Trypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by ultra-filtration.
Items | Specification | Method |
Description | white crystalline lyophilized powder | EP6.0 |
Identification | A) A purple colour develops B) No colour develops with in 3 min of mixing | EP6.0 |
Appearance of solution | Solution S is not more opalescent than reference suspension 3 | EP6.0 |
Absorbance | 1) 280nm : 13.5 ~ 16.5 2) 250nm : not more than 7.0 | EP6.0 |
PH | 3.0-6.0 | EP6.0 |
Histamine | Not more than 1µg/0.2µkatal | EP6.0 |
Loss on drying | Not more than 5% | EP6.0 |
Limit of trypsin | Not more than 1% | EP6.0 |
Microbial limits Total viable aerobic count Salmonella E Coli | ≤10000cfu/g Negative Negative | EP6.0 |
Assay | Not less than 0.5 ukatal/mg, calculated on the dried basis | EP6.0 |
Storage
Sealed, Dark, at temperature 2-8°C
Trypsin 0.5ukatal/mg