Fermenter/fermentation tank/fermentor/bioreactor NameDF-BED (FIXED-FLUIDIZED BED) FIBER DISK CELL CULTERE BIOREACTOR -GLASS TYPEModelCELLIPOWER08 system DF-BED/GFeatureShanghai RITAI is pleased to introduce to the world their first DF-Bed (Fixed-Fluidized Bed) unit for fiber disk cell culture. DF-Bed unit is developed base on the principle of fluidization, consisting of a primary fixed bed with a secondary fluidized bed. The process allows fiber disks to periodically and briefly flow up and rotate in the media solution. The duration and period can be customized. The DF-Bed cell culture retains all benefits of Fixed Bed cell culture while overcoming its disadvantages, increasing cell density and production. This process avoids the impact of mechanical shearing force. Cells are grown inside the disk, cultivating good colony effect. It can perform direct aeration, air bubbles are rotated and dispersed, increasing the concentration of oxygen in media solution. It can perform sampling without contamination, cell counts and monitor cell growth. And it is excellent potential for scale up.ApplicationIt is suitable for anchorage independent and anchorage dependent cell culture, and it is good for serum free cell culture, long time perfusion processing, harvesting the metabolized products;Description

Model	CELLIPOWER08 SYSTEM
Working volume	5L,10L,15L
Filling volume	65-80%
Material	Special type of magnetic silicone glass , which can resist temp. and pressure. Wetted parts are SS316L, and others is SS304.
Stainless steel part finish	inside surface is 0.28-0.6μm by mechanical polishing, ( passivation or electropolish are optional) outside surface is 0.4-0.8μm by satin finish.
Pressure	ATM (atmospheric pressure)
Agitation	1.Fermentor use servo motor 2.top mechanical agitator , single mechanical seal 3.speed range:50-400rpm 4.FIXED-FLUIDIZED BED type agitator
Equips of vessel	1.using alcohol flame inoculation 2.electrical heater 3.PH,DO, temp. sensor foam and level probe port, etc.
construction	bench-top One fermentor vessel with one control system (simens PLC and HMI)
Operating panel	Chinese and English version
Main components and controls of system
PH	1.On-line check and measure, and digitization fully automatic control; 2.PH, CO2 and base gang control. Mass flow meter automatically adjusting CO2, peristaltic pump automatically adjusting base. 3.Control range 2-12pH, measure precision±0.01 pH, control precision±0.1 pH; 4. It has auto alarm function. 5.pH sensor and signal transmission line, mettler Toledo
DO	1.On-line check and measure; digital display. 2.Range 0-200%, precision ±1%; 3.It has auto alarm function; 4.DO sensor and signal transmission line, mettler Toledo
Feed controlperfusion	1.Automatically control and automatically alarm; 2.peristaltic pump automatically feeding and perfusion
Temp. control	1.PID fully digitization signal processing, digitization control settings 2.Measure range 0-150°C, control range 15-45°C,control precision±0.2°C 3.Pt 100Ωtemp. Sensor 4.system with electrical heater to control the temp inside vessel
Rotate speed	1.Automatically control , with auto alarm function 2.servo frequency converter to adjust the speed.
Air flow	Automatically adjusting with massflow meter
sterilization	Should sent to autoclave for sterilization
Control	Siemens PLC, and Siemens 10.4” LCD touch screen(HMI) and RITAI program
PC	1.PC with kingview software for recording function (optional) 2.PC with simens remote control and kingview recording function (optional) 3.printer (optional)

cell culture bioreactors