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Cardiac troponin I (cTnI) is one of the three sub-units, and also the only myocardial contractile muscle protein fine silk, in systolic and diastolic myocardial process plays an important role. The molecular weight of cardiac troponin I to 22.5KD. Myocardial injury cTnI easily released in circulation. In skeletal muscle troponin I also exists, but cardiac troponin I in the N-terminal 31 additional amino acid residues, in the skeletal muscle of this isomer is non-existent, making a cardiac - specific troponin I.
Acute myocardial damage, cTnI immediate access to blood circulation, a process that typically last seven to 10 days. In the event of acute myocardial infarction (Acute Myocardial Infarction, AMI) 4 ~ 6 hours of sensitive immunological methods can be detected in the blood of cTnI. CTnI in normal serum concentrations of less than 0.06ng/ml AMI patients blood cTnI can be as high as 100 ~ curve. Students Lantier ZBLJ cTnI-S-Detection Kit is a qualitative measurement of serum cardiac troponin I rapid detection kit. The reagent can be together with other diagnostic methods used to assess myocardial infarction induced myocardial injury.
Detection Principle CTnI reagents used solid-phase chromatography immune technology. Through the process of detection of cTnI reactor hole in the sample by adding 2 to 3 drops about 80 ~ 100 μ l serum them. Color samples spread through attachment of particles coated membrane antibodies, antibody attached to the beginning and curing contact with the antibody, serum cardiac troponin I concentrations greater than 0.3, in the detection of cTnI particles are cTnI antibody-capture, and there a color zone. CTnI higher concentrations detected in a band of faster, more ribbons deep. Color coated particles of antibodies to control the spread of the catch was the second antibody in contrast to the band. Students Lantier cTnI Kit adjusted to normal serum testing positive will not occur.
Kit components In a statement Single packaging × 25 per single package containing: ZS-cTnI-S-1 reactor A plastic straw A desiccant packet
Reactor Stability The original reactor at room temperature (2 ° C to 25 ° C) storage. See the period of validity from kit label.
Steps Before the operation carefully read manuals. 1. Remove packaging from a single reactor. Detection devices will be reflected on the stage. Available to room temperature. 2. When necessary, use distilled water to dilute the positive standard freeze-dried to HepG2, and then used to dilute negative serum concentration of low-standard goods, negative standard without dilution. 3. Samples will be available to room temperature or standards. 4. 80 lessons with a plastic straw μ l ~ 100 serum samples of fresh or standard, by adding reactor hole in the sample.
Results Sample after 10 to 15 minutes to read the result. Over time to read the results of a false positive risk. Serum samples of high-viscosity, and in the background clear response after reading the results.
Positive: a detection zone in the line of control zone in a clear line of control (= 2 red line that cTnI sample concentration> 0.3). The following map:
Negative: Not a testing line, with only the control line (= control a red line that cTnI concentrations below detection kit for the critical value), the following plans:
Through a series of sample results judgment, we can see that with the concentration of serum samples cTnI increase in the intensity of band increased. If students Lantier cTnI reactor and the results of other methods and symptoms consistent with the patient, that patients with myocardial infarction. When patients with suspected myocardial infarction, it is recommended to patients at different time from the series of samples done.
If the result is negative and with the results of other methods not at the same time, there is a need for re-determination after the first hour. If the result remains negative, and samples used by patients in the sample, the incidence has more than six hours, unlike patients with myocardial infarction. Generally positive results in a few hours or days remain unchanged. If the results need archiving, laboratory safety can be called chromatography removed from the reactor after preservation
Sensitivity When the standard cTnI soluble in human serum concentrations greater than 0.3ng/ml, use of the reactor Lantier cTnI can be detected positive results.
Specificity Installation of this reaction by skeletal muscle calcium containing eggs (sTnI), troponin C (Mi) and troponin T (cTnT), serum, no cross-reaction.
Note 1, the kit is only used in vitro measurement of serum cardiac troponin I (cTnI). Reaction devices and processing specimens in accordance with the general rules and laboratory attention. During the testing within 10 to 15 minutes should not touch the reactor. Two, every reactor in just one. Proofreading batches before use and validity. Do not use outdated equipment and the standard reaction. The use of packaging is not damaged reactor. If a batch reactor with a positive control line, the reactor can not be used for diagnosis
3, in order to avoid a false positive result, the need fresh blood sample within three hours of use. Needless to temporarily take in the sample after sample preparation immediately frozen, use complex to melt at room temperature before using. Second samples can freeze-thaw. 4. In the final diagnosis, not to rely solely on a certain test results, patients should combine the integrated considered. 5. The new batch of kits before use, or other circumstances so warrant, to preferences for quality control testing
cTn I test cassette