High-Pressure Gas Gene Gun:
1. It is a all-new gene-delivery technology, also a physical method, in which the genetic material or other
substances attached to a high-speed microbomb are shot directltly into the cells,
2. It is a late gene-transferred technology internationally.
3. It can make a wide range of cell types instantly stable and highly-efficient transformation.
4. It has a special rupture which generates a gas shock ware.
5. The degree of Helium-pressure that drives the carrier film is determined by the different rupture
filmsused in the process.
6. Within a proper atmospheric pressur the different rupture films penetrate the DNA-coated microcariers into
the target cells at the bottom of the bombardment chamber.
7.The gene gun is applicable in gene-transformation of plants and animals, cell cultures, embryos, bacteria and
small animals.
Main Technical Parameters and Application:
Experimental conditions | In vitro, from the body, plants (in vivo) |
Target region | (big)40cm2 |
Pressure range | 3-12MPa |
Target Type | Animals: cell and organ culture Plants: The smaller of the whole plants, cell culture, explant, yeast, bacteria and other micro-organelles, chloroplast, mitochondria |
Voltage | 220V/110V |
Gas | Helium or nitrogen ( helium is more otimized) |
Metal Particle | Φ0.6-1.6μm, (Common Φ1μm) |
High-Pressure Gas Gene Gun