Identity Specifications
Dehydrated Appearance | White powder, free-flowing, homogeneous |
Solution | 5.0% solution, soluble in purified water upon boiling. Solution is colorless. |
Prepared Appearance | Colorless solution. |
Reaction of 5.0% Solution at 25°C | pH 7.4 ± 0.2 |
Cultural Response
Prepare the medium per label directions.
Staphylococcus aureu | Uniform turbid growth, precitptation at tube bottom, “+++” |
Streptococcus pyogenes | Turbid pricipitation gorwth, “+——++”. |
Shigella sonnei | Uniform turbid growth, “+++——++++” |
Salmonella typhimurium | Uniform turbid growth, “+++——++++” |
Pseudomonas aeruginosa | With plaque, “+++”. |
Escherichia coli | Uniform turbid growth, “++++” |
Formulae
Approximate Formula* Per Liter
Peptone ....................................................... 10.0 g
Beef extract ................................................... 3.0 g
Sodium Chloride ............................................ 5.0 g
Usage
Common used in general bacterial multiplication culture and subculture.
Nutrient Btoth