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| Item | Method | Specification |
| Sensory Test | ||
| Appearance | visual check | deep orange powder |
| Odor | compare to standard | characteristic marigold odor |
| Color | visual check | yellow orange |
| Sieve Analysis | sieving | 100% pass 40mesh |
| General Analysis | ||
| Lutein | UV | ≥2% |
| Residual Solvents | GS | ≤200ppm |
| Lead (Pb) | GB 8449 | ≤2ppm |
| Arsenic (As) | GB 8450 | ≤2ppm |
| Cd | ≤1ppm | |
| Hg | ≤0.1ppm | |
| Microbiological analysis | ||
a. Total plata count b. Mould and yeast c. E.Coli d.Salmonella | ≤1000cfu/gm ≤100cfu/gm Negative Negative | |
Poultry feed additive