... beadswill be absorbed onto the wall of the tube, then suck all the liquid ( it mustbe fully-sucked). The ... vibrate it 15 times to mix up well ( or suck the liquidslowly 15 times to mix up well), leave ... separation to absorb the beads onto the wall oftube, then suck all the liquid ( it must be fully-sucked). 5 ... ~ 100µL eluting buffer into the EP tube, suck the liquid slowly for 15 times to mix upwell, ...