... µL lysis buffer into a 1.5ML centrifuge tube ( EP tube ); point vibrate 15 times, one second each time ... tank to incubate 10---15 minutes. 2, Take the EP tube out from the tank, add 300µL combining ... 3 min. 3, First time magnetic separation:hold the EP tube in the left hand, hold the rectangular magnet ... 65°C ) water tank for 10min. Slightly shake the EP tube several times every 2---3 minutes.7, If not ...