... L lysis buffer into a 1.5ML centrifuge tube ( EP tube );point vibrate 15 times, one second each time, ... water tankto incubate 10---15 minutes. 2, Take the EP tube out from the tank, add 300µL combining ... min. 3, First time magnetic separation:hold the EP tube in the left hand,hold the rectangular magnet ... 65°C ) water tank for 10min.Slightly shake the EP tube several times every 2---3 minutes. 7, If ...